When obtaining a sample from a 0.1 to 10 microliters (ul) pipette, there are two types of tips available: soft and hard. The former has a large button next to the plunger, which you push to release the tip. Once you have finished using the 0.1-10 microliters pipette tips, simply grab the base of the tip and remove it from the liquid. Then, dispose of the unused part in a waste container or autoclavable garbage bag. Remember that residual bacteria on the tip will present a biohazard, and that it should be disposed of properly.
To determine how much sample to dispense, you must first determine the 0.1 microliters/milliliters pipette tip's graduation. It will be filled up to the top of the pipette if the volume of the solution is ten-thousandths of a milliliter. Similarly, a 0.2-ml (M1) micropipette will contain 0.025-mL of solution.
Micropipettes come in different standard sizes. Usually, micropipettes can measure volumes from 0.1 microliters to a million microliters, which equals 1 milliliter. Each type of a pipette has a specific range of volume measurements. The manufacturer will label the plunger with its maximum and minimum volumes, which are usually the same.
Despite the different sizes of the tip, they all share the same range of measurement. A P2 reading, for example, is equivalent to 1.52 mL of solution, while a P200 reading will dispense one milliliter of solution. There are also some that indicate tenths or hundredths of a microliter in red.
The number of a 0.1-10 microliters micropipete is indicated by the dial numbers on the tip collar. There are also variations in volume between the P2 and P200 tips. If the P200 reading is marked 152, the corresponding volume is 15.22 mL. Similarly, a P200 reading of 152 signifies one hundredth of a milliliter of solution is equivalent to 1.22 mL.
The micropipettes of Sarstedt have a jagged design on the tip collar and are made from virgin polypropylene. These micropipettes are more efficient as they require 40% less material to produce and are therefore more environmentally friendly. They are also 100% performance-certified. The sensitivity of these products is reflected in their high accuracy.
The micropipettes are available in various sizes. However, most are available in a range of 0.1 to ten microliters. Among these, the P2 has the highest sensitivity and a P200 has the lowest sensitivity. The graduations represent a standard laboratory condition. The actual results may vary slightly. They are often not representative of the real world.
The 0.1 to ten microliters size range allows you to use a single pipette to collect liquids of various concentrations. The ten microliters range is the smallest range in which a sample will be produced. The smallest volume of a 0.010-microliters tip is used for testing in the laboratory.
The P2 and P200 metric graduations indicate how much of the tip is filled. The P200 marking represents the amount of 0.1 microliters. The other type of ten microliters tips are available in a range of 0.2-ten microliters. The size of a metering pipete depends on the concentration of the sample.
The 0.1-10 microliters pipette is the most popular in the molecular biology lab. The 0.001 mL volume can be filled with a 0.05 mL tip, which is equivalent to a milliliter. The P100 and P200 are both ideal for analyzing DNA samples. The white and yellow tips are used in diagnostics.