Using gloves, lab-coats and hoods does just that. Use your lab-coats only inside your cell culture lab and have them cleaned often. Make sure your hood is serviced often to guarantee that it is properly working. Having a hood is not enough to ensure a sterile environment: you must use it correctly. First, make sure […]
Leave the distilled water out for about 15 minutes to ensure temperature consistency. Use the thermometer to measure the temperature of the distilled water. Record. Place the empty, clean beaker on the balance. Tare the beaker’s weight. Ensure the pipettes are clean, unclogged and ready to function. When filling the pipette up to the volume […]
It's easy to measure your pipetting accuracy – just pipette the same volume of water ten times onto a tared balance, note the weight each time. Then measure the relative standard deviation in the dispensed masses by calculating the standard deviation and expressing it as a percentage of the average. Calculate the accuracy of the […]
Air displacement pipette An air displacement pipette is a common laboratory tool used to handle a measured volume of liquid between 1 µl to 1000 µl (1 ml). Due to its high accuracy, this laboratory tool is commonly used in standard pipetting applications. An air displacement pipette has six main components (plunger button, tip ejector […]
12 tips to improve your pipetting technique in 2021 As your laboratory’s demand for accuracy and precision grows, so does the importance of understanding and developing an optimal pipetting technique. Walk through the following 12 steps that will bring your pipetting to the next level, and see how many you have already mastered and integrated […]
To calculate the accuracy you can use the equation A = 100 x Vavg/V0, where A is the accuracy of the pipette, Vavg is the average calculated volume and V0 is the value you set the pipette to dispense. Accuracy should be between 99-101%. Perform at least 4 measurements each at 100 % and at […]
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. Check if your pipette […]
