When using micropipettes, it's important to know the proper depth for your tips. A small amount of fluid in a micropipette tip can improve accuracy by up to 5%, but if the tip is submerged too much, the volume of the liquid will be compressed or take up too much space. Also, the tip should never be immersed in liquid. Typically, the proper depth for your tip is one to two millimeters, and three to six mm in a large volume pipette.
Ensure that the pipette tip is properly rinsed and coated with the sample to increase accuracy. When using a micropipette, squeeze the plunger slowly and gently. For best results, pipette the liquid at room temperature. The temperature can affect the volume of a sample, so it's important that the liquid, tip and pipette are the same temperature. Use a pre-rinsed tip for viscous, foamy, or small volumes.
When using a micropipette, always remember not to submerge the tip in the sample. Failure to do so can cause inconsistent results, which could result in skewed results. As a result, it's important to use proper pipette tips to avoid mistakes and maximize accuracy. However, there are some precautions that should be taken when using a micropipette.
For consistent accuracy, you should use high-quality tips with your micropipette. Using brand-specific tips will provide consistent results. However, it's okay to use non-brand tips if they are proven to work with your specific pipette model. Inaccuracy and imprecision can occur when mismatched pipete and tip are used. Moreover, a quality tip provides an airtight seal with no excessive force.
A high-quality micropipette tip should be used with a pipette that is compatible with the tips. In most cases, you should use the manufacturer's brand-specific tips, but if you're not confident with a particular brand, it's okay to use an alternative brand if it has proven to work with your model of micropipette.
You should never submerge a micropipette tip in liquid. A mismatched micropipette tip will not perform as well as you would expect. This is why it's essential to pre-rinse the micropipette tips before using them. Using the correct type of pipette tip will ensure a consistent, accurate pipetting process.
If you are pipetting viscous liquids, volatile solvents, or ultra-micro samples, you should always use the reverse method. For both types of pipettes, it's necessary to push the piston to the "purge" position and hold the tip of the micropipette tip in the aspirate position. Then, you should push the piston back to the "aspirate" position and release the remaining sample from the micropipette tip.
When pipetting viscous liquids or volatile solvents, it's important to use the reverse method. This method will ensure that the tip will reach the reagent or solution at full volume. In addition to the reverse method, you can also use the forward method. This method allows you to release the entire volume of reagent or solution from the tip. The fluid will be released from the tip.
When pipetting viscous liquids, volatile solvents, and ultra-micro samples, reverse pipetting is a useful technique. You can simply push the piston to the "purge" position and release the entire volume from the tip. In contrast, in standard mode, you should hold the tip in the aspirate position to prevent reaspiration. By doing so, you will be able to inject the sample to the destination tube without having to remove the micropipette tip.
When using micropipettes, make sure to use high-quality tips. Although manufacturers typically recommend the use of their own tips, it's acceptable to use other brands as long as they are compatible with the pipette model. Otherwise, mismatched tips can result in inaccuracy and imprecision. The best micropipettes have a reliable airtight seal and are free of any mold defects. During the aspiration process, the plunger should be pushed at a constant speed.