Look at the front face of the pipet and you will see a window with three digits inside. The diagram below shows the MAXIMUM value that can or should be dialed in on each size pipet. To exceed these values will put the pipet out of calibration. Beside each "window" below is the numbers place it represents. Please take the time to learn how to read them so as to avoid damaging them by dialing values out of their range. Always select the SMALLEST size pipet that will handle the volume you wish to move to achieve the greatest accuracy. Accuracy decreases as you use unnecessarily large pipets for small volumes.
Start studying Micropipette. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Micropipette. attach a clean disposable tip on the end of the micropipette before use. Nice work! You just studied 5 terms! Now up your study game with Learn mode. RULE OF THUMB: Always select the SMALLEST size pipet that will handle the volume you wish to move to achieve the greatest accuracy. Accuracy decreases as you use unnecessarily large pipets for small volumes. The diagram below shows the MAXIMUM value that can or should be dialed in on each size pipet. To exceed these values will put the pipet out of calibration. Beside each "window" below is the numbers place it represents.
The nominal volume of a variable volume micropipette is the maximum volume of the range specified by the micropipette manufacturer. The purpose of the present study is to quantify the reproducibility standard uncertainty for non-nominal volume micropipette calibrations for variable volume micropipettes. An interlaboratory study was implemented for this purpose, using variable volume micropipettes with nominal volumes of 10 μL, 200 μL, and 1000 μL and setting the measurement volumes as 10 %, 50 %, and 100 % of the nominal volumes. In our previous paper (Accredit Qual Assur 19:377, 2014), we quantified the uncertainty due to reproducibility using only data obtained for nominal volume calibrations.
The pipettes you have may not look exactly like this one, but all the basic parts are the same. Always be sure the pipette you're using is appropriate for the volume you're transferring, and never tilt the pipette when there's liquid in the tip (it can flow into the pipette barrel).
In a way, pipettes work a lot like drinking straws in that they allow liquids to be 'sucked-up' into one end. They are used to accurately measure and transfer small volumes of liquids. Though they may work like drinking straws, never use your mouth to suck-up liquids into a pipette! Instead use a pipette-aide as described below. Pipette-aides or pipettors are suction devices that are used to either suck liquids into or expel liquids out of pipettes. For some types of measurements it may be necessary to expel, or blow-out, the total liquid volume from the pipette using the pipette-aid. Types of Pipette-Aids. a. Least expensive type of bulb; not easily controlled. b. A pipette pump that can be used to take up and expel liquid. c. More expensive bulbs allow fine control of liquid. This type of pipette aid may be called a "triple valve" device because it has three buttons: The first displaces air from the bulb, the second is used to draw liquid into the pipette, and the third is used to expel the liquid. d. Electronic pipette aid that allows fine control and ease of use.
Micropipettes are the type of air displacement pipettes that are used to transfer the small quantities of solutions measured in microliters, 1000 µl (microliters) equals to 1 ml (milliliter) and is the maximum amount that is commonly dispensed with the micropipettes.
The air displacement micropipettes work on the common air displacement principle. A plunger is depressed by the thumb and as it is released, liquid is drawn into a disposable tip. When the plunger is pressed again, the liquid is dispensed.