Molecular biology is the branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including molecular synthesis, modification, mechanisms, and interactions. The study of chemical and physical structure of biological macromolecules is known as molecular biology. Molecular biology is a specialized branch of Biology and Biochemistry, which are specifically concerned with the study of various biological activities at the molecular level. Molecular biology includes different biomolecules like the AminoAcids, Nucleic acids (DNA and RNA) proteins, carbohydrates, and lipids, along with their compositions, interactions, structure, and functions in the life processes. The term "Molecular Biology" was coined by an American scientist, Warren Weaver in the year 1938.
The Micropipette: An Overview: Micropipettes are probably the most used tool in the laboratory. They are used to transfer precise, very small volumes of liquid. Their units are generally measured in microlitres (μL) which is one thousandths of 1 mL. The micropipette in the Biotechnology 101 Kit is a 2-20μL adjustable micropipette, so you can set its volume between 2 and 20μL. There are several parts of interest: The micropipette has a pipetting button (1), and an eject-plunger (2). There is a dial that shows the currently set volume (3). Finally, there is the front of the pipette, onto which the pipette tip (4) attaches.
A micropipette is a small glass capillary with an internal diameter smaller than that of a cell. In this technique, the micropipette is extended to the surface of a cell, and a small negative pressur. A Micropipette is a standard type of laboratory equipment that is used to measure the small volumes of liquids. Micropipettes are also used to transfer a small volume of liquids accurately from one vessel to another. Adjustable micropipettes are more complex than a simple disposable pipette.
Here is how to use a micropipette. For transferring a measured amount of liquid micropipette tips need to be attached with the shaft of the micropipette. Liquids are never drawn directly to the shaft. Different colored tips are available. Their color specifies the volume limits they can measure. White tips are used for P2, P10, and P20. Yellow-colored tips are used for P100 and P200. Blue colored micropipette tips are used for P1000. The tips are present usually in a sterilized form in a plastic box with a cover. The cover should be closed to prevent any chance of airborne contamination.
Pipettes and micropipettes are used to measure and deliver an accurate volume of liquid. Pipettes are used to measure small volumes starting from 1 millimeter whereas micropipettes are used to measure the very small volume of liquid usually in microliters. Micropipette works by displacing the air from the pipette shaft and allow the liquid to be drawn into the resulting vacuum. Pipettes and micropipettes are very essential tools used in microbiology labs, medical labs, and environmental sciences for various academics and research studies.
The micropipettors in this laboratory come in three different sizes each of which measures a different range of volumes. The three sizes are P20, P200 and P1000. These sizes are noted on the top of the plunger button.
Micropipettes can be mainly categorized into two types – Air Displacement Micropipettes and Positive Displacement Micropipettes. You can choose the right one by matching their applications to the experiment you wish to perform.
Eppendorf pipettes are a common laboratory tool used to measure and transport liquid. Due to the cost of these useful instruments, you'll have to take care to preserve their condition. Pipettes also have different colored tips which represent different dosages.
Micropipettes work by air displacement. The operator depresses a plunger that moves an internal piston to one of two different positions. The first stop is used to fill the micropipette tip, and the second stop is used to dispense the contents of the tip.