As your laboratory’s demand for accuracy and precision grows, so does the importance of understanding and developing an optimal pipetting technique. Walk through the following 12 steps that will bring your pipetting to the next level, and see how many you have already mastered and integrated as part of your everyday pipetting routine. 1. Pre-wet the pipette tip. 2. Immerse the tip to the proper depth during aspiration. 3. Pause consistently after aspiration. 4. Use consistent plunger pressure and speed. 5. Pull the pipette straight out. 6. Examine the tip BEFORE dispensing a sample. 7. Examine the tip AFTER dispensing a sample. 8. Use standard mode pipetting. 9. Use the appropriate pipette. 10. Use the correct pipette tip. 11. Work at ambient temperature equilibrium. 12. Minimize pipette handling.
The following is a list of eight helpful tips for your lab to consider, based on 25 years of servicing pipettes for Cambridge customers. These techniques are intended to further improve your pipetting practices, and ensure the most accurate results for your critical assays. Use consistent force when installing the pipette tip. Condition, condition, condition. Aspirate at 90 degrees, dispense at 45 degrees. When aspirating, place the tip just below the surface. Release plunger slowly. Dispense against the side of the receptacle. Touch off at the very end of your receptacle. Don’t hold the pipette when you’re not pipetting.
Pipettes are tools widely used in the lab and usually purchased with care. However, as pipette tips are only consumables, they are not usually selected with quality in mind. The standard ISO 8655 requires an extra calibration if pipettes and tips from different manufacturers are used. This study including tips from 15 different manufacturers proves that a pipetting system working perfectly with a certain tip exceeds the permissible error tolerances when a tip from a different manufacturer is used. Furthermore, we found that the calibration method influences the performance of the pipetting system: It is significant whether the calibration is done with or without tip change for each measured volume. Autoclaving impacts the tip dimension as well as the calibration result especially with small volumes. Eppendorf standard tips have been shown to perform within permissible error tolerances regardless of the calibration method or autoclaving.
While using a pipette, the precision and accuracy of even the best-calibrated pipettes can get eliminated if the wrong kind of tip is chosen. Depending on the nature of your experiment, if the tip is not compatible, it can also make your pipette a source of contamination, resulting in the waste of precious samples or expensive reagents. Advantages Of Pipette Tips: Liquid handling is a significant part of laboratory experiments. Though it is a hassle-free process when handling bigger volumes, it can become a challenge when analyzing minute quantities in microliters. That is when Pipette tips come to help. Here is how Pipette tips can make liquid sample handling a lot easier and accurate.
Pipetting errors are a common hazard of lab work that contribute to variation within and between experiments. Most handheld pipettors are air displacement pipettors. They work by focusing air pressure through your pipette tip so that no mechanical part of the pipette touches your sample. Air displacement can be a highly precise method of liquid handling that protects samples from contamination. However, factors that impact air pressure or pipette tip function can lead to volume errors, particularly when working with small volumes.
The filters prevent aerosols from reaching the pipette body and potentially contaminating subsequent samples. Always change the pipette tip after each sample. Regularly autoclave, or disinfect, the pipette or the components that may come into contact with the sample.
If you work at the bench, accurate pipetting is crucial. Without accurate pipetting, your experiments wouldn't be reproducible, your stock solutions would be inaccurate, and your assays would have such large errors that comparing them would be meaningless.
Failure to choose the proper tips for a given type of pipette can lead to an inadequate seal between the pipette and tip, causing leakage and sample loss.
Any undesired variations in these quantities can jeopardise results and so it is essential to take all steps necessary to maximise pipetting accuracy and precision and reduce the level of uncertainty.