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How Should You Avoid Cross Contamination of Samples When Using a Pipette?

Posted on May 11, 2022 by Pipette Tip 
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How To Prevent Cell Culture Contamination

Scientists can also treat cultures to reduce the odds of contamination. "Antibiotics are a common method to prevent bacterial and yeast contamination." Inside the body, cells are protected by the highly evolved and sophisticated immune system. Once isolated from tissues, cells in culture are "on their own". We must therefore protect them with careful aseptic techniques to prevent their contamination from microorganisms that are present all around us, including inside the laboratory. In order to prevent cell culture contamination, you should wear gloves, lab-coats and use hoods. This goes without saying, you must provide a barrier between your cells and the non-sterile environment that is your laboratory (or indeed yourself).

thermo-finntip
thermo-finntip

Preventing Contamination in Pipetting

Many things can be done to prevent contamination when pipetting. The main things to consider when pipetting is to employ proper pipetting technique, using the proper pipette tips and making sure pipettes and pipette accessories are properly cleaned and maintained. Preventing contamination in pipetting is paramount to achieving reliable results. It requires identification of the potential contamination mechanisms in order that they can all be addressed. Preventing contamination in pipetting is paramount to achieving reliable results. It requires identification of the potential contamination mechanisms and understanding why it is important to prevent contamination, in order that they can all be addressed.

Laboratory #1 – Pipette and Micropipette Reagents/Supplies

Make sure that the tips, the micropipette and the reagent/solution are at the same temperature. Forward Pipetting Technique: To aspirate the liquid in the tip, press the plunger to the first stop. Immerse the pipette tip vertically in the liquid. Slowly release the plunger while the tip is immersed. The liquid will be aspirated into the pipette tip. This pipette is a general purpose pipette for sampling and dispensing accurate liquid volumes. The pipettes operate on the air displacement principle and use disposable tips. The black tip cone can be sterilized 121℃, 0.15Mpa.

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thermo-filter-tips

Ways to Avoid Cell Contamination: Top Pipetting Tips

How to avoid it: Use filter pipette tips or positive displacement tips to prevent samples from entering the pipette body. While pipetting, hold the pipette in a vertical position. Store pipette in an upright position. Tilt the bottle while pipetting. Avoid splashes of samples from entering the pipette by slowly releasing the button. Contamination of the pipette itself can be avoided by using manufacturer recommended pipette tips of proper length and employing slow dispensing methods to avoid splashes. When not in use, contamination can be avoided with regular cleaning and proper storage stands.

Pipette Tip - Laboratory Equipment

Pipette Tips are disposable, autoclavable attachments for the uptake and dispensing of liquids using a pipette. Micropipettes are used in a number of laboratories. A research/diagnostic lab can use pipette tips to dispense liquids into a well plate for PCR assays. Universal Biologicals Pipette Tips ABclonal's sterile and non-sterile pipette tips are able to extract and dispense specific volumes by releasing them from a partial vacuum. General Pipette Tips Non-barrier or general tips are designed for everyday use in the lab, especially when working with chemicals that will not contaminate your pipette. Non-filter, non-barrier, general or standard tips are used for many non-sensitive applications. These tips are generally used when sterility is not important. Faced with COVID-19, pipette tip shortage is a threatening problem.

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thermo-cliptip

FAQ of Pipette Tips

What is the purpose of a pipette tips?

Pipette tips are small, disposable tips that are made of molded plastic. Shaped like cones, a scientist attaches a pipette tip to the end of a pipettor and then uses it to transfer the liquid. The liquid used in the pipette tip is measured in microliters (μL), or one-thousandth of a milliliter (mL).

How do pipette and burette differ?

The key difference between burette and pipette is in their release mechanism. Burettes have a stopcock at the bottom while a pipette has a dropper like system that releases liquid in the desired amount by lessening the vacuum.

thermo-art-tips
thermo-art-tips

Why is it important to swirl the contents during titration?

This is because there is no more acid in the flask to react with the base that has been added. To get the most accurate measurement of how much base is needed to react with the acid, we need to ensure the solutions are continuously mixed together well so that they can fully react.

https://youtu.be/wiGUAh-_sUY

What precautions should be taken during titration?

Usually an air bubble is present in the nozzle of the burette,it must be removed before taking the initial reading. Carefully wash down with distilled water any drop of the solution that sticks by the side of the conical flask during titration.

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tecan-pipette
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