Tip Loader - A new way pipette tip refill, save time, save money, reduce waste 

How to Check the Accuracy of Your Pipette?

Posted on March 31, 2022 by Pipette Tip 
 Tip Loader For Pipette Tip Refill  
tip loader for pipette tip rack loader
For Laboratory use
Tip Loader For Pipette Tip Refill 

The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs. Check if your pipette needs to be calibrated: Learn how to calculate pipette accuracy and precision to compare the values obtained with the specifications. Pipette accuracy testing is a verification process performed by the user. This guide explains the principles of a pipette test.

thermo-fisher-cliptip
thermo-fisher-cliptip

10 Steps to Improve Pipetting Accuracy

Pre-wet the pipette tip. Aspirate and fully expel an amount of the liquid at least three times before aspirating for delivery. Examine the pipette tip for droplets. Pause consistently. Minimize handling of pipette and tip. Use the correct pipette tip. Common techniques for avoiding drips and improving pipetting accuracy include: Reverse Pipetting; Pre-wetting the tip; Anti-Droplet Control.

thermo-scientific-art-tips
thermo-scientific-art-tips

Pipetting Best Practices

Proper Pipetting Angles – Angles play a critical role in both maximizing pipette performance and minimizing user errors. Pre-wetting — After installing a tip onto your pipette, aspirate the select volume of liquid and dispense it back into the source container. Aspiration Depth — During each aspiration step, it is critical to submerge your pipette tip no more than 1-2mm below the surface of the liquid to avoid adhesion. Consistent Smooth Speed and Pressure — While aspirating and dispensing liquid, use a smooth controlled speed through to the first stop and through to the blowout.

https://youtu.be/fyOV9iyZMNY

Pipette Calibration

What is pipette calibration? It is a process of examining and/or adjusting the pipettes to the true value by comparison to the standard which is set by the manufacturer of that said pipette. The standard in this case is the pipetting range set by the pipette manufacturer to be accurate and precise at low volume and high volume of the pipette. Pipette calibration table of Z factors for distilled water at 1 atm pressure Source: Wisconsin State Laboratory of Hygiene Steps Involved in Pipette Calibration.

thermo-art-tips
thermo-art-tips

Pipette Accuracy vs. Precision | Thermo Fisher Scientific

A pipette is accurate to the degree that the volume delivered is equal to the specified volume. Precision, on the other hand, is concerned with the closeness of several measurements to each other, rather than to a standard value, that is the reproducibility of the pipetting samples.

sterile-tips
sterile-tips

FAQ of Pipette Tips

How do you find the error of a pipette?

Subtract one value from the other: 2.68 - 2.70 = -0.02. Depending on what you need, you may discard any negative sign (take the absolute value): 0.02. Divide the error by the true value:0.02/2.70 = 0.0074074. Multiply this value by 100% to obtain the percent error.

thermo-finntip
thermo-finntip

Why pipette is more accurate and precise?

The long, narrow, and slender neck of the volumetric pipette makes it easier to measure and read the meniscus very precisely. As opposed to the measuring cylinders, volumetric pipettes also prove beneficial while dealing with potentially harmful substances because of the way they hold the samples.

Is there a difference in precision of pipettes?

When pipettes are both accurate and precise the mean volume is the set volume and there is no variation between different pipettings.

rainin-pipettes-price
rainin-pipettes-price

What is the accuracy of pipette?

A pipette is accurate to the degree that the volume delivered is equal to the specified volume. Precision, on the other hand, is concerned with the closeness of several measurements to each other, rather than to a standard value, that is the reproducibility of the pipetting samples.

thermo-fisher-art-tips
thermo-fisher-art-tips

What is accuracy in pipetting?

Accuracy is the quality of being true, correct, exact, and free from error. Accuracy is the ability of a pipette to give a response close to a true or nominal volume as indicated by the volume setting.

Hot Sales
Tip Loader
For laboratory use
Pipette Tip Sorting Device
For tips manufacturer
tip-loader.com

Contact Us

Write Us Today
Reply Whitin 8 Hours

Tip-Loader.com

Tip Loader is pipette tip sorting device for bulk pipette tip refill into empty rack.
We use cookies in order to give you the best possible experience on our website. By continuing to use this site, you agree to our use of cookies.
Accept
Privacy Policy