Pipetting whole blood Use forward technique steps 1 and 2 to fill the tip with blood (do not pre-rinse the tip). Wipe the tip carefully with a dry clean cloth. Dip the tip into the blood and press the operating button to the first stop. Make sure the tip is sufficiently below the surface. Release the operating button slowly to the ready position. Guide to Pipetting. Pipetting is at the core of many experiments. That's why the Gilson Guide to Pipetting is a must-have reference for every lab. Filled with tips from our pipetting experts, this free guide covers all aspects of pipetting, from pipette selection through maintenance. Immerse the tip to the proper depth during aspiration Before aspirating, immerse the tip adequately below the meniscus. Large volume pipettes (1-5 mL) should be immersed to 5-6 mm, while smaller volume pipettes should be immersed to 2-3 mm. Too little immersion, particularly with large volume pipettes, can lead to air aspiration.
Pre-wet the pipette tip Aspirate and fully expel an amount of the liquid at least three times before aspirating for delivery. Failure to pre-wet the tip increases evaporation within the tip air space, which can cause significantly lower delivery volumes. 1. Prewet the pipette tip; 2. Work at temperature equilibrium; 3. Examine the tip before and after dispensing sample; 4. Use standard mode pipetting; 5. Pause consistently after aspiration; 6. Pull the pipette straight out; 7. Minimize handling of the pipette and tip; 8. Immerse the tip to the proper depth; 9. Use the correct pipette tip; 10. Use consistent plunger pressure and speed.
The filter pipette tips serve the basic purpose of protecting pipettes from aerosols and aspirating volatile or viscous solutions, avoiding contamination and damage of the pipette. The filter pipette tips are pre-sterilized, and are fitted inside the proximal part of the pipette tip. Filtered pipette tips must be used in all molecular biology applications that are sensitive to contamination. The filter tip helps reduce the possibility of smoke formation, prevents aerosol contamination, and thus protects the pipette shaft from cross-contamination. Filter tips feature the unique safety space air gap, so your sample never touches the filter. They offer optimal protection for your work from cross contamination and enable you to use the full volume of the tip with any pipetting mode.
The precision and accuracy of even the best calibrated pipette can be wiped out if you choose the wrong kind of tips. Depending on the experiment you are doing, the wrong kind of tips can also make your pipette a source of contamination, lead to waste of precious samples or reagents—or even cause you physical harm in the form of repetitive stress injury (RSI). Aerosol Barrier tips, also known as filter pipette tips, have a filter inside the proximal portion of the tip. Only a few high-end tips similar to Hamilton filter tips offer an excellent sealing barrier. Due to the presence of barriers in these tips, they are ideal for delicate applications such as qPCR.
The filter pipette tips, also known as the aerosol barrier tips, serve the basic purpose of protecting your pipettes from aerosols and aspirating volatile or viscous solutions, avoiding contamination and damage of the pipette. The filter tips are pre-sterilized and are DNase/RNase-free, and are fitted inside the proximal part of the tip. Filter tips protect pipettes from the aerosols that may build up while aspirating fluids using a pipette. Non-filter Tips Non-filter tips are made of high molecular material polypropylene without bending.pipette tips wall is silicified, and the inner wall is smooth without wall hanging. DNase/RNase free, non-pyrogenic.
Basically, the main influencing factors are design/shape, production quality and material. As shown, these factors do not only influence the single pipetting result, they also have methodical consequences like varying results when calibrating with one/several tips or changing results by autoclaving.
Three different types: Pipette tips come in three different types including non-sterile, pre-sterilized and filtered tips. The most commonly used type of pipette tip is non-sterile tips. They are often used in laboratory applications where sterility is not important to the experiment or test being performed.
Pipette tips allow scientists to measure specific amounts of liquid in their experiments. With pipette tips, you can zero in on the details and get as precise as possible in your measurements. This, in turn, allows for more accurate results.
Pipette Tips are disposable, autoclavable attachments for the uptake and dispensing of liquids using a pipette. Micropipettes are used in a number of laboratories. A research/diagnostic lab can use pipette tips to dispense liquids into a well plate for PCR assays.