How do you find the volume of a micropipette? On the body of the micropipette is a small vertical window with three numbers. These numbers, read from the top down, tells the user how much volume the micropipette is currently set at. To change the numbers, grasp the black dial or plunger and gently twist. The numbers will then change. The proper technique to have while using a micropipette Select the volume. Set the tip. Press and hold the plunger at the first stop. Place the tip in the liquid. Slowly release the plunger. Pause for a second and then move the tip. Insert the tip into the delivery vessel. Press the plunger to the second stop.
Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman. Each pipetman has a three-digit volume indicator, but the (slightly) tricky part is that the digits mean different things depending on whether you're looking at a P20, a P200, or a P1000. This means, incidentally, that the top number on the number dial can only be set to a few digits. On a P20 or a P200, it can only be set to 0 or 1. On a P1000, you have to leave it at 0. If you "overload" the number, you won't actually cause any explosions, but you will damage the calibration of the pipette.
The P-20 is calibrated in 0.1 μl increments, the P-200 in 1μl increments and the P-1000 in 10 μl increments. To adjust the volume, hold the pipet with one hand and with the other turn the volume adjustment knob (black ring) so that the appropriate number appears in the indicator window. Transferring liquids up to 1 ml: Pipettor P-20, P-200, P-1000 Micropipetting: Most pipettes come in three sizes: P-20 for dispensing 1-20 μl, P-200 for 20-200 μl range, and P-1000 for 200-1000 μl range. The P-20 and P-200 pipets use the same disposable tip (usually yellow) that attach to the white stem. The P-1000 uses a larger tip (blue).
Measurement range of each pipette: P2 - .2 - 2; P20 - 2 - 20; P200 - 20 - 200; P1000 - 100-1000. What is important to note about the range of measurement for each pipette: Setting the pipette to measure below or above its threshold will result in inaccurate measurements and may damage the instrument. What is important to note about the dials for setting the desired measurement: Each pipette will read with the same numbers, but different valued pipettes will represent different values.
The underlying technical and operational knowledge of micro-pipetting is essential for scientists, technicians, and students to achieve precise results from their experiments in Biomedical Sciences and other related disciplines. Since it is one of the crucial prerequisites for Good Laboratory Practices (GLPs), the key fundamentals of the micro-pipetting methods, such as forward and reverse pipetting and the importance of ergonomics and maintenance etc. Basically, the micropipetting techniques are classified into two main types, namely, Forward Pipetting and Reverse Pipetting.The first step in micropipetting will be to adjust the micrometer in the pipette to set the volume required based on the assay protocol.In the second step, an appropriate disposable tip will be attached by gently pushing the pipette shaft over a tip, and the plunger button will slowly be pressed to the first stop outside of the solution to displace air and avoid blowing bubbles into it.
Numbers on the micropipette (typically black-black-red) are read as XX. X µl. The change in color indicates the position of the decimal point. P20: Maximum volume 20 µl.
In a P100, the top number refers to 1000's of µl, the middle number refers to 100'sµl and the bottom number refers to 10's of µl's. In a P200, the top number refers to 100's of µl, the middle number refers to 10's µl and the bottom number refers to µ'ls.
Micropipettes are a crucial tool for them to transfer volumes of samples into a small cavity (called a well) in the gel. Today, you will practice using a micropipette by loading colored samples into wells.
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs.
They are used to transfer precise, very small volumes of liquid. Their units are generally measured in microlitres (μL) which is one thousandths of 1 mL. The micropipette in the Biotechnology 101 Kit is a 2-20μL adjustable micropipette, so you can set its volume between 2 and 20μL.
