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How To Use a Micropipette?

Posted on March 31, 2022 by Pipette Tip 
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Micropipettor: Operation and Applications

Air displacement micropipettes operate by piston-driven air displacement. When the piston is pressed downwards, the air within the sleeve of the micropipette gets expelled out due to the force of which the liquid present in the micropipettes tip also gets removed. Positive displacement micropipettes operate by piston-driven displacement. The piston in a positive displacement micropipette is in direct contact with the liquid. When the piston is pressed downwards, the liquid which is present in the sleeve of the micropipette also moves downwards and gets removed through the tip. When the piston is pulled upwards, it also draws the liquid along with it in the upward direction.


How to Use a Micropipette

Fixed volume micropipets often do not have an tip ejector button, so the tips are removed manually. The Experiment Procedure: You will use a variable micropipette to prepare seven different dye mixtures in the wells of a microtiter plate. Steps to follow when using a micropipette 1. Select the volume 2. Set the tip 3. Press and hold the plunger at the first stop 4. Place the tip in the liquid 5. Slowly release the plunger 6. Pause for a second and then move the tip 7. Insert the tip into the delivery vessel 8. Press the plunger to the second stop 9. Pause for 2 seconds 10.Remove the tip 11.Release the plunger 12.Eject the tip into the waste container


Mastering the micropipette

Fill stroke Depress the plunger to the first stop Insert the pipette a few millimeters into the solution to be transferred Slowly release the plunger up to the starting position (tip will fill with fluid) For the most accurate transfers, keep the micropipette within 20 degrees of the vertical axis. Therefore, in every experiment, you will be required to accurately measure volumes as small as a few microliters (μL). Micropipettes will allow you to do this accurately and precisely. 2.1: Using micropipettes correctly. 2.2: Using the spectrophotometer to evaluate your pipetting skills. 2.3: Light spectroscopy.

Pipette | Biochemical Methods

Using a Pipette: Pipettes are omnipresent in the biochemical methods lab. They allow us to measure volumes of liquid precisely and accurately and are an integral part of most experiments we will be performing. In this activity, you will learn (or be reminded of) how to use a pipette in the proper fashion. Take the pipette and push the button slowly to feel the two different stops. The position of the first stop is directly related to the volume setting. Set the pipette to 5μL and feel the position of the stops. Now set the pipette to 20μL and note the difference. Pipettes are used to transfer small quantities of liquid. They provide efficiency and accuracy and are especially useful for repeated transfers. All adjustable pipettes should be calibrated and checked periodically. The simplest way to accomplish this is to pipette a specific volume of a solution with a known density (remember density = mass (g) / volume (mL)). The density of distilled water is 1.0 g/mL.


What Is the Purpose of a Pipette?

A pipette is a small tube that can transfer liquids from one container to another. Pipettes, or pipets, are common pieces of laboratory equipment. Types of Pipettes Pipettes may be constructed out of glass or plastic and are used to transfer a measurable amount of liquid. Pipettes provide a clean and hygienic environment to aspirate, transport and dispense liquid samples. The purpose-built tools help to maintain quality assurance in the laboratory and ensure the most reliable and accurate results possible. Modern pipettes often feature disposable tips which helps to minimise the risk of cross contamination.


FAQ of Micropipette Tips

What is adjustable micropipette?

Adjustable Micropipette: It is easy to use, highly accurate and uses standard micropipette tips. The volume is easily selected by twisting the top. The lightweight design and tip ejector makes operation fast & easy.


Are micropipettes fixed or adjustable?

Unlike adjustable volume pipettes, fixed volume pipettes dispense the same quantity of liquid every time you dispense. The volume of a fixed volume micropipette cannot be changed, which allows the user to work at a faster pace without having to constantly change the volume setting on the side or top of the pipette.


What are the different types of micropipettes?

Micropipettes can be mainly categorized into two types – Air Displacement Micropipettes and Positive Displacement Micropipettes. You can choose the right one by matching their applications to the experiment you wish to perform.


Why are micropipettes so expensive?

These pipettes are expensive because the barrel and the tip are a unit and both are replaced when pipetting. A cheaper alternative is to use an air displacement pipette with barrier tips, but these only address some of the problems.


What are the four styles for micropipettes?

 4 Commonly Used Lab Pipette Types:

  • Air displacement micropipettes. The most common micropipettes use air displacement to aspirate and dispense liquid. ...
  • Positive displacement pipette. Positive displacement pipettes use piston-driven displacement to aspirate and dispense liquid. ...
  • Electronic pipettes. ...
  • Multichannel pipettes.
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