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How to Use the Various Types of Pipettes?

Posted on March 30, 2022 by Pipette Tip 
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Graduated pipette

A graduated pipette is one type of pipette that allows the user to determine the volume of liquid that is being removed. Pipettes and micropipettes are chemical droppers used to measure and dispense exact amounts of liquids. This instrument is also referred to as a Mohr pipette, after Karl Friedrich Mohr, a 19th century pharmacist. Fisherbrand™ Disposable Graduated Transfer Pipettes: Ideal for hematology, urinalysis, chemistry, and blood banking — anywhere there is a need for quick, safe transfer of fluids. Thermo Scientific™ Samco™ Graduated Transfer Pipettes: Measure more than one volume accurately by using these graduated polyethylene transfer pipettes. Built-in pipette bulb eliminates need for plugging, packaging and autoclaving. Thermo Scientific™ Samco™ Transfer Pipettes: Prevent cross-contamination with these transfer pipettes, which have a built-in bulb that eliminates the need for plugging.

finntip-pipette
finntip-pipette

The proper use of Pipettes and Pipette-aids (Pipettors)

Pipetting aids should be selected with care. Their design and use should not create an
additional infectious hazard and they should be easy to sterilize and clean. A pipetting aid should always be used. Pipetting by mouth is prohibited. All pipettes should have cotton plugs to reduce contamination of pipetting devices. Plugged (aerosol-resistant) pipette tips should be used when manipulating microorganisms and cell cultures. Air should never be blown through a liquid containing infectious agent. Infectious materials should not be mixed by alternate suction and expulsion through a pipette.

pipetman-diamond-tips
pipetman-diamond-tips

Introduction to Serological Pipettes and Pipettors

The serological pipette is a laboratory instrument that transfers liquids measured in volume by ml. Most have graduations on the side for measuring the liquid being dispensed or aspirated. An important step when culturing or plating cells is the even distribution throughout a solution. The serological pipette It is a type of sterile pipette that is mainly used for cell culture and / or work with sterile solutions. It is a type of graduated pipette also called terminal pipette, since the graduations occupy the entire surface of the pipette, including the tip. This differentiates it from the rest. The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable.

Types and Uses of Serological Pipettes

Serological pipettes are primarily used in the following: Mixing suspensions. Combining reagents and chemical solutions. Transferring cells for empirical analysis or expansion. Layering reagents for creating higher density gradients. Serological pipettes are designed to be used as blow-out pipettes. The use of serological pipettes is an efficient and gentle means of mixing cell suspensions. They can also be used for mixing reagents and chemical solutions. After treatment or isolation of experimental cell cultures, serological pipettes are useful in transferring colonies of cells for further empirical analysis or expansion.

pfact-filter-tips
pfact-filter-tips

3 Different Types of Serological Pipettes

There are three different kinds of serological pipettes, all of which serve a different purpose in testing and experimenting: Open-End Pipettes; Bacteriological Pipettes; Aspirating Pipettes. Open-End Pipettes: The Open-end pipettes are specially designed pipettes that feature an open-end for use with high viscosity liquids. Bacteriological Pipettes: Used primarily in the examination of dairy products, milk or bacteriological pipettes are designed for use in the dairy field and meet the American Public Health Association (A.P.H.A) standard for the Examination of Dairy Products. Aspirating Pipettes: Unlike open-end and bacteriological pipettes, aspirating pipettes are completely transparent and do not have gradations.

FAQ of Pipette Tips

What are serological pipettes?

Serological pipettes are temperature calibrated laboratory tools that are the ideal choice for laboratories that need to transfer large amounts of liquid (1 mL to 50 mL).

tarson-filter-tips
tarson-filter-tips

Why is a serological pipette more accurate?

Each pipet has clear ascending and descending graduations marked on the side. This ensures accurate measurement when dispensing or aspirating.

sapphire-low-retention-filter-tips
sapphire-low-retention-filter-tips

Why would you use a serological pipette?

The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable.

What is the difference between a serological and a measuring pipette?

The key difference between volumetric and serological pipettes is that volumetric pipettes are calibrated to deliver a certain volume of a solution through free drainage, whereas serological pipettes are calibrated all the way to the tip, and the last drop of the solution has to be blown out.

sorenson-pipette
sorenson-pipette

Are serological pipettes sterile?

Designed for accurate and rapid fluid dispensing, serological pipettes are sterile, pyrogen-free, cotton plugged, and can be used for a variety of liquid handling applications (certified DNase/RNase free). They are manufactured from transparent medical grade polystyrene and sterilized by gamma radiation.

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