How to wash sepharose pipette tip? The first step in washing sepharose is to dissolve the magnetic beads using a 1:1 slurry of PBS and BSA-blocked protein A sepharose 6MB. This stock should be agitated for a few seconds so that the beads settle evenly. Afterward, the tips should be rinsed with distilled water. The final step is to confirm that the amount of radioactivity is within the acceptable limit before disposing of the used materials.
To decontaminate the pipette, clean the lubricant with a solution of 70% ethanol in DEPC-treated water. Autoclaving is the easiest method for sterilization, and it should be done according to the instructions provided by the manufacturer. To sterilize the tip, place it into an autoclave and sterilize for 20 minutes at 121 degC.
In addition to cleaning, pipettes should be disinfected. Sodium hypochlorite is effective at removing DNA and RNA contamination, but you should also check the label of your equipment to ensure that the tip is compatible with the chemicals you are using. Lastly, pipettes should be washed by immersion in distilled water, and you should use a chemical-free solution to remove any traces of the product.
If you do not have access to distilled water, you can use a chemical disinfectant. This is the easiest way to sterilize a pipette. It is a quick process, and you should read the instructions on the packaging carefully. Alternatively, you can use a commercial sterilising solution. If you're unsure about what type of solvent to use, you can use an ethanol-based sanitizer.
To sterilize a pipette, you should follow the manufacturer's instructions. For example, if you are using a PCR solution, you should sterilize it with sodium hypochlorite. However, if you don't use a PCR solution, you can use distilled water to clean the pipette. If you are sterilizing DNA, you should soak the pipete in a solution of sodium hypochlorite.
When you're cleaning your sepharose and pipette tip, make sure they're both clean and free of debris. After disassembling the pipete, wash the tip and the tube with distilled water. For RNA, you can dip the pipette into sodium hypochlorite to kill any traces of DNA. This solution is also useful for removing RNA from the pipette, which is easily degraded by ubiquitous RNases.
Once the tips have been thoroughly washed, you can reuse the tips. After you've used them, you can reload them with fresh ones. You should use a fresh ethanol bottle to rinse your pipettes. Afterwards, you should treat the tips with Thermo Scientific DNA AWAY Surface Decontaminates to kill any remaining DNA or RNA. Then, you should store them in the refrigerator to ensure that they don't cross-contaminate.
After washing, you should rinse the pipette with distilled water or ethanol. Sodium hypochlorite can kill DNA, but you should also sterilize your pipettes by using Thermo Scientific DNA AWAY Surface Decontaminates. It's important to use a sterilizer, which is the simplest method. If you're using the autoclavable version, place it in a closed bottle and wait 20 minutes at 121°C.
You should wash the pipettes with a detergent solution, such as Deconex(r) 12 Basic. After cleaning, you should rinse with distilled water and repeat the procedure for any soiled tips. Thermo Scientific DNA AWAY Surface Decontaminates can be used to treat pipettes and tips. If you're concerned about DNA contamination, you can soak them in NaOCl for a while to destroy it.
After completing the PCR, you can then proceed to washing the pipette tip. For a better result, you should wash the pipette tip in a solution of 6% NaOCl. This solution will eliminate any trace of contamination from the pipette and the sepharose. In some cases, the solution is not suitable for a further PCR.