The most obvious reason to eject all liquid from pipette tips is to ensure a clean delivery system. However, sometimes the reverse technique is required, which is less ideal when a solution has a high viscosity or foams easily. In such a case, a reverse technique is more appropriate. Regardless of how you eject liquid from a pipette tip, you must make sure that the tip is free of all debris.
When ejecting liquid from a pipette, it's best to use a volumetric pipette. These are designed to have little remaining solution in the tip because of surface tension. When using these types of pipettes, be sure to squeeze the cap tightly to release all liquid. After using the volumetric tip, let it drain out to the bottom of the vessel.
A volumetric pipette should never be used for a blowout. If you want to get the full volume of the liquid, you should hold the tip against the side of the container and push the plunger to the first stop. Press the eject button on the tip. Always ensure that you use a new sterile tip for each pipette and use a clean one between samples.
In order to avoid contamination, you must be careful not to eject all the liquid from a pipette. It can cause the liquid to leak out of the tip. So, it's best to use a clean and sterile tip. To avoid contaminating the sample, make sure you eject all liquid from a volumetric pipette before removing it from a vessel.
If you do not have a volumetric pipette, it is not recommended to use a backscratcher. In such cases, it is necessary to use a new sterile tip before proceeding with the experiment. A properly-cleaned tip is also safer, since it is not prone to contamination. So, never eject all of the liquid from a volumetric pipette.
When pipette tips are ejected, they can cause a lot of problems. Firstly, you should be aware of the fact that volumetric pipettes are not meant to be blown out. Because of surface tension, they will have some liquid remaining in their tips. Therefore, when you're using a volumetric pipette, you should not squeeze it to make it empty completely.
When using a volumetric pipette, you should make sure that you do not squeeze the tip as this may eject too much liquid. Instead, you should allow the liquid to drain out of the pipette's tip by releasing the cap and letting the liquid drip out of the bottom. This will allow the remaining liquid to drain out of the tip.
Volumetric pipettes are designed not to be blown out. They should be squeezed and the liquid should drain out from the bottom of the pipette. It's not necessary to squeeze the tip, since the volumetric tip is calibrated according to its volume. It should be firmly on the mouth. If you do squeeze the tip, you will end up with a dripping tip.
The volumetric pipette should be slid gently against the inner wall of the tube at a 30-40-degree angle. Slowly squeeze the tip until the liquid is ejected. The tip should be firmly held to avoid spills. The cap should be tightly on the mouth of the pipette to prevent accidental ejection. When you eject the liquid from a volumetric pipette, you should let the liquid drain out of the bottom.
There are also disposable micropipette tips available. These are usually supplied with an ejector button that can be pressed to remove the tip. The ejector should not eject the liquid, which can lead to contamination and a biohazard. In such cases, eject all the liquid from a pipette and discard it. If the volume of the sample is higher than the recommended volume, it is important to replace the micropipette.
