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What Are Pipette Tips?

Posted on November 11, 2021 by Tip Loader 
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Pipette Tips are autoclavable disposable accessories for taking and distributing fluids with a pipette. A variety of laboratories make use of micropipettes. Pipette tips can be used in a research facility to pour liquids into a well plate for PCR experiments. Micropipette tips may be used by a microbiology laboratory evaluating industrial items to distribute testing materials such as paint and caulk. Pipette Tips are constructed of molded plastic and are transparent so that the contents may be seen easily. Micropipette tips can be non-sterile or sterile, filtered or unfiltered, and devoid of DNase, RNase, DNA, and pyrogens.

10-ul-pipette-tips
Filter Pipette Tips

Pipettes and pipettors employ pipette tips to speed up processing and decrease cross-contamination. They come in a range of materials and styles. The following pipette styles are commonly used: universal pipette tips, filter pipette tips, and low retention pipette tips

It's challenging to think that tiny, plastic-molded disposable tips constitute the lifeblood of molecular biology, chemistry, and medicine. These pipette tips can help you establish a durable and precise pipetting system. Pipette tips are classified into three types: non-sterile, pre-sterilized, and filtered—the amount of microliters that each tip can store ranges from 0.01ul to 5mL.

Non-sterile pipette tips are the more often used form of the pipette tip. They are frequently employed in laboratory settings when sterility is not critical to the experiment or test. This pipette tip is ideal for labs with limited resources. When purchasing bulk pipette tips, you may save a lot of money. Universal pipette tips are intended to accommodate a wide range of pipette types. To be cautious, you must always double-check the size of your pipette's barrel, but generally, universal pipette tips should suffice. However, to achieve decent results, you must ensure that your pipette tips are of excellent quality, so do some study before purchasing.

Pre-sterilized pipette tips, on the contrary hand, are aimed at preventing contamination. These pipette tips are appropriate for sterile purposes such as cell cultures since they are verified free of DNA, RNase, ATP, and pyrogens. Low retention is an excellent feature to be in your pipette tips. They hold relatively more minor liquid, assuring that little to no fluid remains inside the tip after pouring—this aids in the accuracy of readings. When using a conventional pipette tip, some liquid is occasionally left within after dispensing. This is not an issue with low retention pipette tips. They are constructed of a hydrophobic substance, which prevents fluid from clogging the pipette tip.
Filtered pipette tips are intended to prevent the formation of aerosols. Aerosol particles are tiny semisolid particles that float in the air. These particles may linger in the air for lengthy periods and be ingested. Worse, aerosols cause 65 percent of all laboratory illnesses, mainly by breathing them.

Filtered pipette tips limit the possibility of aerosol formation in the laboratory. They also keep pipette shafts clean and decrease the likelihood of cross-contamination. They have a filter in the tip to prevent dangerous liquids from reaching the barrel and messing with the experiment. Pipette tips like this are frequently employed in contamination-sensitive applications like forensics and diagnostics.

We can't stress enough how crucial it is to ensure safe work standards in the laboratory, especially with the danger of infection via aerosols. This involves cleaning pipettes after use and appropriately dumping pipette tips.

Why Are Pipette Tips Essential?

  • Accuracy and precision

Precision and accuracy are essential in every laboratory testing. As a result, each substance or chemical in the assay must be measured as precisely and accurately as feasible to provide the best outcomes. Pipette tips come in handy in this situation. Pipette tips enable scientists to quantify liquid levels in their research precisely. Pipette tips allow you to focus on the details and be as accurate as needed with the measurements. As a consequence, the results are more reliable.

  • Simple to Use and Understand

Pipetting is a method that all researchers should be familiar with. Because of the importance of this skill, even high school students acquire fundamental pipetting abilities. Nonetheless, despite its significance, it is extremely simple. It is a valuable and straightforward skill to learn how to utilize pipettes and pipette tips. Any newbie may master the pipetting skill with the correct pipettor suggestions.

  • Sterile

If you're conducting a sterile experiment, such as cell culture, ensure that you're using pipette tips. They not only decrease cross-contamination, but if you use the appropriate tips, you'll get superior results. Non-sterile pipette tips are frequently used because not all scientific study requires sterility. To preserve your test free of contamination, you may obtain pre-sterilized pipette tips that don't include DNA, RNase, ATP, or pyrogens. This promotes more precise and improved results.

  • Efficacious

Scientists place a high priority on the accuracy, particularly in the laboratory. Pipette tips enable scientists to conduct scientific tests in a timely and efficient manner. They minimize the necessity for more complex, time-consuming apparatus. Although pipette tips are intended to be disposable, they can be reused to promote productivity and sustainability. This, however, is dependent on the tip's quality, your cleaning approach, and the nature of your experiment. For instance, if your test must be kept sterile, discarding pipette tips instead of reusing them is preferable.

  • Diverse

There are no two lab tests that are alike. Each will employ a unique set of circumstances, resources, and approaches. Pipette tips offer a significant benefit in this situation. Pipette tips exist in a variety of shapes and sizes, and there are several pipette tip applications. So, no, despite whatever your test is, it's nearly a certainty that you'll be able to obtain the particular type of pipette tip that you want.

Can We Reuse Pipette Tips?

Pipette tips can be reused for pouring the same solution. Their legibility is also affected by the following factors.

  • The pipette tips' integrity.
  • The substance that you are pipetting.
  • The cleaning procedure you're employing

Summary

Pipette tips are essential in lab studies such as PCR, DNA separation, and purification because they require exceptional accuracy to handle minute fluid amounts. Again, the proper pipette tips are critical in scientific studies. On the other hand, employing incorrect tools might distort findings, which can have devastating consequences for the experiment. If your pipette tips aren't acceptable or compatible with your pipettors, it might have a significant impact on your experiment. It not only skews your results but also wastes time by requiring you to repeat the entire process. To assist you, here is an outline of the relevance of pipettor tips and how they play an essential part in laboratory studies.

Refferences:

Chen, M., Vial, M. L., & St John, J. A. (2019). 3D-tips: User-friendly mesh barrier pipette tips for 3D-spheroid culture. Journal of Biological Engineering, 13(1), 1–10. https://doi.org/10.1186/s13036-019-0210-3

Olmedo, P., Navas-Acien, A., Hess, C., Jarmul, S., & Rule, A. (2016). A direct method for e-cigarette aerosol sample collection. Environmental Research, 149, 151–156. https://doi.org/10.1016/J.ENVRES.2016.05.008

Turoňová, D., Kujovská Krčmová, L., & Švec, F. (2021). Application of microextraction in pipette tips in clinical and forensic toxicology. TrAC Trends in Analytical Chemistry, 143, 116404. https://doi.org/10.1016/J.TRAC.2021.116404

Vlčková, H., Pilařová, V., Novák, O., Solich, P., & Nováková, L. (2017). Micro-SPE in pipette tips as a tool for analysis of small-molecule drugs in serum. Bioanalysis, 9(11), 887–901. https://doi.org/10.4155/bio-2017-0033

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