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What Are The Different Sizes Of Micropipettes?

Posted on March 31, 2022 by Pipette Tip 
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Micropipette | Introduction

Introduction To Micropipettes: Pipettes and micropipettes are used to measure and deliver an accurate volume of liquid. Pipettes are used to measure small volumes starting from 1 millimeter whereas micropipettes are used to measure the very small volume of liquid usually in microliters. A micropipette starter kit is beneficial as it saves the time of selecting the micropipette and the compatible tips which can be used with it. Microlit Micropipette Starter Kit combines 4 single channel pipettes to cover the complete pipetting range (0.5-10ul pipette, 10-100ul pipette, 100-1000ul pipette, 1-10ml pipette).

yellow-tips-for-micropipette
yellow-tips-for-micropipette

Micropipette Tips

Using a micropipette in your laboratory Micropipettes are utilized in the laboratory to transfer small quantities of liquid, usually down to 0.1 uL. They are most commonly used in chemistry, biology, forensic, pharmaceutical, and drug discovery labs, among others. Micropipettes are pipettes capable of measuring and dispensing liquid solutions in the volume range of 1 µL to 1,000 µL. They are commonly used in life science-related research where the accurate measurement and dispensing of solutions is required. A micropipette is commonly used under a microscope to microinject liquid directly into a cell. It is made of glass and has a microscopic tip. Tip apertures can be as narrow as 0.1 µm. Air-displacement pipettes, also called air-cushion pipettes, are used in life-science and other liquid handling labs to precisely measure and move liquid.

yellow-micropipette
yellow-micropipette

Micropipette Tips – 1-10 µl (M3112)

Description Ultra-micro MicropipetteTips, 1 - 10 µl volume range, natural, non-sterile, nuclease free, pk of 250 - For use on all ultra-micro micropipettes, these 1-10 µl polypropylene micropipette tips are supremely clear and precise for classroom and lab use. Micropipette 1-10 µL, Pipettor adjustable single channel Single Channel Pipettes with Superior Accuracy and Reproducibility. … Rubber O-Ring eliminates the need for routine maintenance and lubricationNozzle Designed to fit on Nichiryo original tips as well as many other manufacturer's tips of compatible size.

https://youtu.be/Y4EHLLZm4Xo

Labtip Pipette Tips

Thermo Scientific Labtip standard pipette tips are made from 100% virgin polypropylene. These tips are available in 10, 200, 300 and 1000μL sizes. Available in clear (10μL and 300μL), yellow (200μL), blue (1000μL), these tips for general lab use across countless liquid transfer applications. Labtip pipette tips provide: The most common sizes used in a lab: 10, 200, and 1000μL; Non-Filter essential tips for general laboratory usage; Non-sterile packaging in both Racked and Bulk.

eppendorf-micropipette
eppendorf-micropipette

Micropipette tips sizes

Three of the more common micropipette sizes include P20, P200, and P1000. Each different size is designed to measure liquids within different volume ranges. The P20 micropipette can measure volumes within the range of 0.02 and 0.7 ounce (0.5 and 20 milliliters) while the P200 can measure volumes between 0.7 and 6.8 ounces (20 and 200 milliliters). They micropipette tips are further divided into micro white tips and medium white micropipette tips. Micro white tips are used for P2 and P10 size micropipette used for taking volume from 0.2 µ to 10 µ. Medium white micropipette tips are used for P20 micropipette measuring a volume range of 2 µ to 20 µ.

https://youtu.be/uEy_NGDfo_8

FAQ of Micropipette Tips

How do you read a P1000 pipette?

On a P1000, the most you can measure is 1000, so the top digit is 1000's of microliters (also known as milliliters). This means, incidentally, that the top number on the number dial can only be set to a few digits. On a P20 or a P200, it can only be set to 0 or 1. On a P1000, you have to leave it at 0.

vwr-200-ul-pipette-tips
vwr-200-ul-pipette-tips

How do you read a P100 micropipette?

In a P100, the top number refers to 1000's of µl, the middle number refers to 100'sµl and the bottom number refers to 10's of µl's. In a P200, the top number refers to 100's of µl, the middle number refers to 10's µl and the bottom number refers to µ'ls.

rainin-pipettes
rainin-pipettes

How do you read a P2 micropipette?

If you are using the P2 pipette, the top number represents the ones digit, the second, red number represents the tenths digit, and the bottom, red number represents the hundredths digit. In the example on the left, . 5ul of liquid would be pipetted. The numbers should range from 020 to 200 with this pipette.

How would you set the volume to pipette 20 with the P20?

Set the desired volume by turning the centrally located rings clockwise to increase volume or counterclockwise to decrease volume. P20: Maximum volume 20 μl. Accurate between . 5 μl and 20 μl.

rainin-tips-vwr
rainin-tips-vwr

How do I know which micropipette to use?

As a rule of thumb, always choose the smallest pipette capable of handling the required volume. This is important because accuracy decreases when the set volume is close to the pipette's minimum capacity. For example, if you dispense 50 µl using a 5,000 µl pipette, you will get rather poor results.

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