Introduction To Micropipettes: Pipettes and micropipettes are used to measure and deliver an accurate volume of liquid. Pipettes are used to measure small volumes. A micropipette starter kit is beneficial as it saves the time of selecting the micropipette and the compatible tips which can be used with it. A micropipette is commonly used under a microscope to microinject liquid directly into a cell. It is made of glass and has a microscopic tip. Tip apertures can be as narrow as 0.1 µm. Using a micropipette in your laboratory Micropipettes are utilized in the laboratory to transfer small quantities of liquid, usually down to 0.1 uL. They are most commonly used in chemistry.
A micropipette is an instrument that is used commonly in the laboratory to transfer the liquid volumes accurately and precisely in the range of microliter. Various types of micropipettes are available for use in the laboratory. Different types of micropipettes offer different functions like single-channel micropipettes are commonly used for performing research for microbiology, cell culture, molecular biology, immunology, biochemistry, analytical chemistry and genetics. On the other hand, multichannel micropipettes are better used for diagnostic tests, kinetic studies, DNA amplification, and molecular screening. Different sizes and designs can be found in micropipette. But, some components of a micropipette remain the same for all variations of this product.
You will be using two types of pipettes during the Biotechnology 101 kit. You have an adjustable micropipette, and a bag of disposable transfer pipettes. Transfer pipettes are made from squishy plastic. They are not designed to measure specific volumes, so they are generally used for larger volumes where accuracy is not so important. ‘Large’ in the context of molecular biology are volumes between 0.2mL and 10mL. The transfer pipettes provided measure 1 – 5 mL. You can change the volume that the pipette transfers by rotating the pipetting button. However, avoid dialling the pipette beyond its limit of 20μL.
Do not try to dial the pipette beyond 20μL, as this might break the pipette or cause it to become unreliable in its volume. Never suck up liquid directly into the pipette! Always use a tip, and make sure that the liquid never gets sucked up beyond the tip into the pipette itself. Do not try to dial the pipette beyond 20μL, as this might break the pipette or cause it to become unreliable in its volume. It is good etiquette to set the pipette to the correct volume before attaching a pipette tip. This is in order to reduce the risk of contaming the tip once it is attached to the pipette.
For adjusting the proper volume rotate the volume adjustment dial present at the top of the micropipette. It can be rotated right or left to decrease or increase the volume. The volume that is going to be measured by micropipette is shown on the digital layout. As the volume adjustment dial is rotated the number on the digital layout is also changed. The volume you have set is shown on the digital readout. Micropipettes are used to measure and transfer the volume of liquid with great accuracy and precision in a laboratory. Accuracy in micropipette measurement is necessary as any discrepancy in volume dispensed can affect the result of the experiment. To ensure accuracy, it is necessary to check micropipette calibration after every week.
Micropipettes can be mainly categorized into two types – Air Displacement Micropipettes and Positive Displacement Micropipettes.
A micropipette can come in one of many standard sizes, and the most common can measure out a volume between 0.1 microliters and 1000 microliters. This is 0.0001 milliliters to 1 milliliter. Just as 1000 milliliters is equal to 1 liter, 1000 microliters is equal to 1 milliliter.
Definition of micropipette: 1: a pipette for the measurement of minute volumes; 2: a small and extremely fine-pointed pipette used in making microinjections.
It is necessary for getting perfect results of different tests or researches. Without perfect measurements of different fluids you cannot get desired perfect results which can be sometimes harmful too. Pipettes are the devices which can help you the most in this whole process of experiments and tests in the labs.
The micropipette was invented in 1957 at University of Marburg, Germany by postdoc Heinrich Schnitger. Frustrated by repetitive pipetting of small volumes using glass micropipettes, Schnitger developed a prototype with a spring-loaded piston and a removable plastic tip for containing liquid.
