Pipettes are necessary lab equipment for dispensing precise amounts of fluids. Pipettes are typically used to pull up and distribute liquid by establishing a vacuum pressure above the container that stores the fluid and deliberately discharging this suction to pull up and distribute the desired volume. Micropipettes are pipettes that distribute from 1ul to 10,000ul, whereas micropipettes release a larger volume. Pipettes are extensively employed in molecular genetics, chemistry, and medical diagnostics because they allow clean and precise fluid handling. Pipettes are available in various forms, including manual and electronic pipettes, and single or multi pipettes, all of which are intended for productivity while dealing with various plates, tubes, and dishes. Producers introduce new, better, and better effective pipettes for a wide range of research and cell culture applications.
Although chemical droppers aren't the initial thing that flows to mind when considering procedures like gas chromatography, they're crucial since they serve a purpose that other lab apparatus doesn't. Plastic pipettes are utilized in most scientific experiments and operations, but glass pipettes can also be employed.
The main distinction between the two is that a latex head will be used at one side of a glass pipette to drag and move a liquid in and out of it. However, using plastic pipettes, you may compress the bigger end of the pipette to do this.
Electronic pipette, which can take many samples simultaneously, provide an option to these classic forms of pipettes. This speeds up, simplifies, and improves the accuracy of sterile saline, quantifying and transferring chemicals.
Standard plastic pipette functions as a vacuum, drawing liquids in and out of the instrument by compressing the 'bulb.' Designated units are correctly listed down the shaft of this kind of pipette, enabling measurement recording straightforward. Milliliters are often used as the unit of measurement on a plastic or glass pipette, typically varying from 0.25 and 1 cm, some electronic pipettes reaching as small as 50 microlitres, which amounts to 0.05 milliliters.
Electronic pipettes are said to be higher precise than regular pipettes. The most recent electronic pipettes have a multitude of various options, allowing you to program them to perform whatever you want them to be doing, and they will suck in the substance to your exact requirements. Even operations like dilution and titration are available as choices, and you can specify how much fluid you want it to pull in if you're limited to precise measurements. You'll also be able to alter the pace at which you pull fluid into and out of the pipette, which is helpful if you're working with a volatile chemical.
This could seem cliche, but reviewing the manual is essential for a better pipette operating in the lab. Each pipette is unique, and functionality might vary even across models of the same type. All lab employees should be familiar with utilizing the pipette tips that are accessible in their workplace. Understanding pipette laboratory instrument usage is a crucial aspect of occupational health and safety and boosts precision, productivity, and dependability.
References:
Chen, M., Vial, M. L., & St John, J. A. (2019). 3D-tips: User-friendly mesh barrier pipette tips for 3D-spheroid culture. Journal of Biological Engineering, 13(1), 1–10. https://doi.org/10.1186/s13036-019-0210-3
Wang, M., Yan, H., Yuan, Y., & Han, Y. (2015). Pipette-tip solid-phase extraction by use of a sol–gel hybrid adsorbent: a new pretreatment strategy for rapid screening of cucumbers for cyanazine and atrazine. Analytical and Bioanalytical Chemistry, 407(4), 1231–1239. https://doi.org/10.1007/s00216-014-8336-0
