A micropipette can come in one of many standard sizes, and the most common can measure out a volume between 0.1 microliters and 1000 microliters. This is 0.0001 milliliters to 1 milliliter. Just as 1000 milliliters is equal to 1 liter, 1000 microliters is equal to 1 milliliter. Each micropipette only works effectively within a certain range, and each volume variance of pipette looks very similar to each other. To avoid confusion, manufacturers label the plunger of the micropipette with the maximum volume it can accurately dispense. Some also add the minimum volume, but many don’t. Due to standardization across manufacturers however, the minimum volume for each micropipette is the same unless otherwise stated.
Do not jam the pipette onto the tip as this may damage the fine tip end cause the liquid beam to be redirected or damage the orifice. Do not hold the pipette in your hand while waiting for the centrifuge, cycler, etc. The humidification of the air inside the tip and pipette prepares the tip for the sample and avoids evaporation when aspirating the transfer volume. This is very important when handling small volumes to avoid the capillary effect that occurs when the pipette is held at an angle. Immerse the tip as little as possible to prevent liquid entering the tip due to the capillary effect. The smaller the tip and volume, the lower the immersion depth. Optimal flow-out of the liquid is guaranteed when the pipette is held at a 45° angle. Contact to vessel wall or liquid surface. A blow-out is mandatory after dispensing low volumes to dispense even the last drop of liquid present in the tip.
It's usually fairly easy to calculate the volume of a liquid in a container with a regular shape, such as a cylinder or cube. All you have to do is use the appropriate mathematical equation to calculate the capacity of the container, then measure the level of the liquid and make the necessary adjustment. There are several devices used in the laboratory for dispensing measured volumes of liquid substances and solutions. In this experiment, you investigated the 10- and 50-mL graduated cylinders, a 25-mL volumetric pipet, and a 50-mL volumetric buret. You dispensed nominal volumes of water from each of these devices, and then checked that the volumes dispensed were correct by determining the mass of the water dispensed. Mass can be determined to much greater precision than can volume and can be used to check the accuracy and precision of the volume dispensed.
A micropipette is a scientific tool used to transfer small, exact volumes of liquid between 1-1000ul. Proper use of a micropipette is essential to successfully carry out a scientific experimentthat requires exact volumes of a reagent during the sample preparation. Micropipettes are most often used in general or organic chemistry, biochemistry, and molecular biology experiments. Adjusting the volume of the pipette is the trickiest part of the micropipetting process. A simple mistake could lead to a large error in the preparation of your experiment. You will need to refer to the volume knob and number windows to accurately input the volume you are going to pipet.
Solution preparation relies upon the precise measurement of volumes to obtain the exact concentrations required. Therefore, the choice of micropipette that is used can determine not only the accuracy - but also the reproducibility of your experiments. We offer a selected range of micropipettes spanning from 0.5 μl all the way up to 10 ml. Each micropipette comes pre-calibrated and tested, to ensure accuracy and reproducibility. Additionally, we have prepared a handy guide on how to properly use your micropipette to get the best results possible.
Set the desired volume by turning the centrally located rings clockwise to increase volume or counterclockwise to decrease volume. P10 micropipette: Maximum volume 10 µl. Accurate between 1 µl and 10 µl. Numbers on the micropipette (typically black-black-red) are read as XX.
Basic parts of a micropipette include plunger button, tip ejector button, volume adjustment dial, volume display, tip ejector, and shaft. They differ in design, weight, plunger force, and overall precision.
Micropipettes are generally used in microbiology, chemistry and medical testing laboratories for the accurate and precise transfer of samples.
Liquid is filled in the tip as per preset micropipette volume. To dispense the liquid, place the tip on the inner wall of the receiving vessel at a steep angle. Slowly press the plunger to the first stop to dispense the liquid. To empty the tip completely, press the plunger to the second stop.
Depress the plunger to the first stop and hold it in this position. Dip the tip into the solution to be pipetted, and draw fluid in to the tip by gradually releasing the plunger. Be sure that the tip remains in the solution while you are releasing the plunger.