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What Do Do If Bubble Forms at the Tip of the Pipette

Posted on March 18, 2022 by Pipette Tip 
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When using a pipette, the tip of the pipette often forms air bubbles. These air bubbles may not be visible, but they can lower the density of the liquid and affect the volume measurements. To reduce the formation of air-filled bubbles, use a stirring rod or a bottle-to-cup method to compact the liquid before drawing it. Alternatively, a distilled water-based solution can be used.

A common mistake is releasing the plunger too quickly. This causes air bubbles to form, which affect the volume of the liquid. To avoid this problem, pipettes must be rinsed thoroughly after every use. It's best to flush them three times with water. Always be aware of the dangers of air bubbles, so be careful not to squeeze the tip too hard or too fast.

Aspirating the air bubble is the best way to remove it. It will not affect the volume measurement. To prevent this problem, it is important to flush the pipette thoroughly after use. This can prevent air bubbles and increase the volume of the liquid being delivered. When pipetting small volumes, the tip should be inserted into the liquid only two to three mm below the meniscus. Excessive immersion will cause the liquid to stick to the tip while too little immersion will cause air bubbles. Moreover, the temperatures in the lab will affect the delivery volumes of the liquid.

Ensure that the pipette is not released too rapidly. Sudden release of the plunger will cause air bubbles to form, which may negatively affect the liquid measurement. To avoid air bubbles, pipettes should be flushed with water after each use, and then pre-wettened before each use. If you find that the bubble is still there, try to remove it.

If the bubble is still present, try to stop using the pipette. Immediately re-press the plunger. Ensure that the pipette is not touching the sides of the container. The side of the pipette can cause air bubbles, which can negatively impact the liquid measurement. Afterwards, you should wash the tip of the pipette and repeat the process.

Another way to avoid air bubbles is to ensure that you do not touch the side of the container when pipetting. In order to prevent air bubbles, use a pre-wet pipette tip and pull it out slowly. A smooth motion can help avoid air bubbles. A gentle motion is ideal for delivering a small volume. Ensure that you do not touch the side of the pipette.

In case a bubble forms at the tip of the pipetted solution, you should follow the above steps to prevent the bubble from forming. If the bubble is caused by the air in the pipette, it is important to avoid evaporation. Aspiration is the process of drawing the liquid through the tip. Dispensing the liquid is a slow process.

If you want to avoid the formation of air bubbles at the tip of the pipette, you need to make sure that the pipette is correctly calibrated. A pipette should be kept clean and dry after every use. A regular flushing process is the best way to prevent air bubbles. If the tip of the pipette is too dirty, you should flush the tip with water. If the liquid is too hot, it will be too hot and create a vacuum, which may cause the bubble to float.

If the bubble is very large, you need to try aspirating the bubble. The air in the pipette can cause the bubble to pop and evaporate. If the bubble is small, you should not worry. Aspirating the bubble can remove the excess liquid. But it is not recommended if the liquid is too warm, as it can affect the volume.

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