A good tip when pipetting is to always make sure that the tip of the pipette is completely submerged in the liquid. Doing so will prevent the sample from sticking to the tip of the pipette and causing a loss of the sample. Most manufacturers sell a pipette tip in a single box and this is enough for all of the projects in the kit.
If you're pipetting a viscous liquid, be sure to submerge the tip of the pipette tip in the liquid just below the meniscus. If the tip of the pipette isn't fully submerged in the liquid, air will be trapped and the volume transferred will be inaccurate. It's also recommended that you hold the pipette vertically when pipetting and keep it there for a few seconds after aspiration.
When pipetting, it is important to ensure that the tip is not too deep into the liquid. This can cause the tip to become compressed and too much liquid to be drawn out. This can lead to inaccurate measurements. During this process, you should hold the tip vertically and do not touch the pipette while opening and closing it. However, if the liquid is too hot, extra droplets can stick to the pipette tip and create an inaccurate measurement.
Before beginning to pipette, make sure the tips of the pipettes are at room temperature. The changes in temperature will affect the volume of liquid dispensed. Furthermore, body heat will impact the accuracy of the results, so you should avoid transferring too much. In addition to avoiding such inaccuracies, pre-wetting the tip will improve your accuracy.
When pipetting a sample, you should make sure to fully submerge the tip into the liquid. This will prevent any unwanted air from escaping into the liquid and prevent the sample from being contaminated. When you have a sample with a high concentration, it is essential to ensure that the tip is sufficiently below the meniscus. Moreover, if the liquid is too thin, the pipette should be held vertically to avoid the liquid from getting out of the tip. The tip should be sufficiently below the meniscus, but should not be too far below the level of the liquid. After aspirating, hold the tip of the pipette against the inside of the vessel. This will draw out the rest of the liquid. Once you have done this, you will know if there is any air in the liquid.
When pipetting, you should hold the pipette plunger at an angle of about 10 degrees from the top of the liquid. It should not touch any sides of the liquid. A slight change in temperature will affect the volume of the liquid dispensed. If you are holding the plunger at an angle, the tip will not be as accurate and may lead to wicking.
During pipetting, the pipette tip should be held vertically and the pipette should be kept in the middle of the liquid. The pipette should be positioned such that it is completely below the meniscus without touching it. During aspiration, the tip should remain in the liquid for a few seconds. Then, the liquid should be poured into the pipette and the tips should be flushed down the drain.
When pipetting, it is important to submerge the tip into the liquid. The ASTM standard specifies that the tip should be inserted sufficiently below the meniscus without being too deep. The tip should be fully submerged in the liquid so that the liquid is adequately dispensed. During aspiration, the pipete should remain submerged in the liquid for a few seconds after aspiration.