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Which Type Of Pipette Is Most Accurate?

Posted on March 30, 2022 by Pipette Tip 
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Selecting the Right Pipette Tip | Thermo Fisher Scientific

The type of experiment you are performing and the physical properties of the liquid will determine the correct pipette tip to use. Badly fitting pipette tips allow air to escape when drawing up and dispensing, thus leading to inaccurate results. Different types of tips are now available in the market - extended length pipette tips that can reach to the bottom of sample vessels without contact by the pipette, solvent safe carbon filtered tips that offer pipette and sample protection against aggressive aerosols and vapors from volatile organic solvents, wide orifice tips designed to eliminate cell sheering and flow resistance, individually wrapped tips for applications requiring stringent aseptic conditions, gel loading tips, sterilized filter tip to prevent aerosol contamination, and low retention tips designed for applications requiring high accuracy and reproducibility.

rainin-pipet-lite-xls-tips
rainin-pipet-lite-xls-tips

Everything You Wanted To Know About Pipette Tips And More!

It is hard to believe that simple, plastic molded disposable tips are the bread and butter of molecular biology, chemistry and the world of medicine. That’s right, we are talking about pipette tips. These tips create a dependable and accurate pipetting system. Pipette tips come in three different types including non-sterile, pre-sterilized and filtered tips. The most commonly used type of pipette tip is non-sterile tips. They are often used in laboratory applications where sterility is not important to the experiment or test being performed. On the other hand, pre-sterilized pipette tips are designed to prevent contamination.

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rnase-free-tips

A Guide to Various Types of Pipette Tips

Non-sterile tips and sterile tips: It is common knowledge that sterile tips should be used for applications where sterility is essential. Long pipette tips: To cut back on this risk, a lot of manufacturers offer extended-length pipettes that are suitable for lab use, like microcentrifuge pipettes or deep ell blocks. Short tips: First, they support the targeting or any small wells, like when manually pipetting into a 384 or a 1536 well plate with multichannel pipettes. Second, they offer improved ergonomics by letting you pipette closer towards the bench, reducing the strain you have on your arm. Wide bore tips: Fragile cellular samples can become damaged when they’re forced through a narrow orifice of standard pipette tips.

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Pipettes and Tips Selection Guide: Types, Features, Applications

There are three main types of pipettes and tips: Pasteur, positive displacement, and air displacement. Pasteur Pasteur pipettes are small glass tubes with a bulb at the end used for dispensing small amounts of fluids. Both Pasteur and positive displacement types of pipettes have a piston that moves in a cylinder, or capillary. Positive Displacement Pipette, is used for applications like PCR and other DNA amplification techniques. The micro-syringe tips used in positive displacement pipettes are disposable. This helps to avoid sample-to-sample cross-contamination (also known as sample carry-over), and contamination due to the aerosol effect.

tips-200ul
tips-200ul

The Importance of Buying the Best Pipette Tips

Pipette tips are in integral part of your liquid handling procedure, and using poor quality or ill-fitting tips will significantly impact the performance of even the best pipette. Linda knows to look for premium quality tips from a reputable vendor that are produced from high-quality polypropylene. When looking to buy a micropipette, it is important to make sure that the pipette is comfortable to hold. This will ensure that you are able to use the pipette accurately and efficiently. Additionally, it is important to make sure that the pipette is of high quality and is able to withstand wear and tear.

sartorius-tips
sartorius-tips

FAQ of Pipette Tips

What are the three types of pipettes?

nnon-sterile, pre-sterilized and filtered tips. The most commonly used type of pipette tip is non-sterile tips. They are often used in laboratory applications where sterility is not important to the experiment or test being performed.

Which type of pipette is most accurate?

Volumetric pipettes. Volumetric pipettes are considered the most accurate, with the capacity to measure up to four significant figures. A volumetric pipette, bulb pipette, or belly pipette [1] allows extremely accurate measurement (to four significant figures) of the volume of a solution.[2] It is calibrated to deliver accurately a fixed volume of liquid.

vwr-200-ul-pipette-tips
vwr-200-ul-pipette-tips

What are the types of volumetric pipette?

Four main types of volumetric glassware are common: the graduated cylinder, the volumetric flask, the buret and the pipet. These have specific uses and will be discussed individually.

sartorius-filter-tips
sartorius-filter-tips

How to handle a volumetric pipette?

Place the tip of the pipet into the receiving vessel and remove your finger to allow the liquid to drain. Touch the pipet once to the side of the vessel. There will be a small amount of liquid in the tip. Most pipets are calibrated to account for this liquid.

https://youtu.be/0ulSRvlhmX8

Why is the volumetric pipette the most accurate?

The main advantage of a volumetric pipette is its accuracy. It is especially precise when it is delivering solutions, as another advantage of the standard build is its narrow neck. This allows for the meniscus to be read more accurately, and therefore deliver more precise results than graduated pipettes.

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