It is essential to use the correct size of pipettes. This is because a smaller tip will deliver a smaller volume of sample. A bigger tip will hold a larger volume of sample. However, it is still crucial to make sure that the pipette is pre-wetted with a solution before using it. A pre-wet pipette tip will ensure that the sample is adequately delivered and that the pipette is as free of air as possible.
Several tips are available to improve the performance of your pipettes. To ensure a high volume of delivery, the tip should be pre-wetted before delivering liquid. If you fail to do so, the liquid will cling to the tip and cause the aspiration to be less efficient. In addition, prewetting the tip will increase the humidity within the tip, reducing the chances of evaporation.
The most common mistake made by amateurs is pressing the plunger to the second stop after aspirating a sample. This is a mistake that can lead to over-pipetting and damaging the tip. Instead, it is best to press the first stop and then reverse the draining process. This will result in the most accurate and precise aspiration. Then, you can use the same pipette for different types of liquids.
Pipettes have a feature called "blowing out volume." This feature allows you to aspirate a certain amount of air after the liquid is in the tip. The air volume is important for preventing droplet formation while it is in transit. It should also be held at an angle of 90 degrees. In addition, it will prevent the tip from touching the walls of the container and will reduce over-pipetting.
Incorrect aspiration can also damage the tip. Standard micropipettes must be held upright when aspirating liquid. An angle steeper than 20o will reduce accuracy. The microPro's angle remains perfectly vertical from plate to plate. A resulting aspiration will be more precise and accurate. The solution for this problem is to reverse the draining of the pipette and fill it with distilled water.
Incorrect aspiration can affect the quality of your sample. For example, incorrectly aspirating a small sample can result in the sample not being aspirated properly. Then, the pipette can jam, which can result in a break in the shaft. Aspiration can also cause contamination and repetitive stress injuries. Choosing the right tip is essential when aspirating a small sample.
Aspiration is a common problem for new users of pipettes. The filter tips in the pipette will protect your samples by preventing carryover and PCR contamination. In addition, a filter tip will give you more robust results. Having a filter tip is a valuable training tool for new lab staff. If a lab member accidentally aspirates a liquid, it will damage the whole tube. Therefore, throwing away the tip is a safer option.
The size of the tip is an important factor in aspiration. It should fit snugly into the pipette's barrel. Otherwise, it will allow air to escape. This is dangerous as it will result in a sample that does not contain the desired volume. You should choose the right tip for your needs. Most manufacturers offer tips for different sizes and styles of pipettes. For optimal results, it is a good idea to buy a universal tip to reduce the chance of miscalibration.
Before aspirating, pre-wetting the pipette tip is critical. A dry pipette will not allow liquid to aspirate. Before aspirating, you should thoroughly wet the pipette's tip with water and then dip it in the liquid. A sample that is too wet will result in under- or over-pipetting. So, when you aspirate, ensure that the sample is completely hydrated.
It is important to follow the ASTM standards when using pipette tips. The ASTM standard specifies that the tip should be submerged at a depth of 20 degrees below the liquid. Any lower than this will cause air to enter the tip and produce a lower volume of sample. If you are unsure of whether the ASTM standard is applicable to your specific application, read the instructions provided by the manufacturer.