If you have ever noticed that your pipette doesn't dispense the volume it displays, you might be wondering, "Why don't I just get a new one?" This problem has many causes. In some cases, the sample doesn't adhere to the tip. In other cases, the sample is partially blocked by air, which results in underdelivery. In such cases, researchers try to compensate by placing the tip deeper into the tube.
Aside from ensuring accuracy, pipette tips are also useful for preventing contamination. A pipette tip should always be moistened before use. Using a dry tip can lead to sample loss due to evaporation. Wiping the tip with a dry rag is also a dangerous practice. This can steal sample volume. It is not uncommon for the researcher to mistakenly think the pipette tip is contaminated when it is dry.
To prevent contamination, pipette tips are usually sterilized to reduce cross-contamination. In addition to preventing contamination, they produce more accurate results. Often used in scientific research, non-sterile tips are used for routine reagents. To ensure better results, use sterile pipette tips, which are free of DNA, RNase, ATP, and pyrogens.
When you use a pipette, it must have a sterile tip. If you are not sure what type to choose, you may want to use a disposable pipette. It will be safe to dispense liquid that is labeled as hazardous or contains other materials.
While pipettes are made of plastic, a plastic one is not as durable as a plastic one. The most common tip that is used is one made of metal and cannot be cleaned with a non-sterile solution. In contrast, a polymer is more flexible and can withstand the pressure of chemicals. A polymer is more flexible than a rubber or plastic pipette. If you use a rubber pipette, you can make it last longer without breaking.
When pipetting liquids, it is important to ensure that the pipette tip is held vertically and in the center of the vessel. After aspiration, the solution should remain in the tip. To collect a sample, hold the pipette plunger at the first stop and then slowly release it. Once you have collected the sample, gently wipe the opening. Then, re-press the plunger until the sample is fully eluted.
A common mistake when pipetting is to use the wrong tip. The wrong tip can lead to a loss of precision. A mismatch of the tip can cause waste of samples and reagents. Additionally, incorrect tips can lead to repetitive stress injury. Choosing the right tip is important when performing experiments. There are several different types of tips for different applications. To avoid the latter, choose a tip with a large diameter and a large number of nozzles.
Using the wrong tip can ruin the precision of a pipette. It can also lead to contamination, waste of reagents, and repetitive stress injury. Properly choosing a pipette tip can prevent these problems. To avoid the risk of a spilled solution, you must make sure that you use the correct one for the desired volume. For example, a silicone-tipped tip is more fluid-resistant.
When you use a pipette tip, it's important to keep in mind that it is not possible to pipette liquid from the tip. The tip is made from plastic and is made to dispense liquid, so you have to use a new, sterile tip whenever possible. The plastic material is very thin and can cause damage to the tip. You should only use a pre-wetted and sterile tip if your volume is more than ten uL.
Pipette tips are crucial for testing Covid-19, which relies on the tip to accurately measure the concentration. This supply chain can be disrupted by freak accidents or natural disasters. In Texas, statewide blackouts forced the closure of pipette plants, which were critical for the test. Then, the supply chain was severely damaged. During a power outage, the tip supply chain was damaged, causing more than a hundred people to lose their jobs.