You may wonder, "Why change the tip on a micropipette when transferring different reagent types?" There are many good reasons. The most important reason is to avoid contamination. Often, people add reagents directly to the pipette, which could result in the contaminated sample returning to the source. Changing the tip is a very simple and safe process.
Micropipettes come with different volume ranges. Some have fixed volume ranges, while others are adjustable within a given volume range. Variable-volume micropipettes have a rotating dial that allows you to adjust the volume range. The manual will explain how to adjust the volume range. While this is an important detail, it's not critical to know the exact steps to change the volume range on a pipette.
To make the transfer easier, micropipettes come with a volume-reduction mechanism. The liquid inside the tip is dispensed or aspirated. If you don't like the consistency of the reagents, you can always change the tip. It's simple to change the volume range with a rotating dial at the top of the micropipette.
Micropipettes are also easy to use and are available in various volumes. Many are equipped with a volume-reduction mechanism. These systems allow you to control the volume in either fixed or variable volume modes. You can also change the tip by using the rotating dial on the top of the plunger. If you are unfamiliar with these features, you should read the manual carefully.
When changing the volume of different reagents, you should always check the volume of the reagents to be transferred. Some reagents have a low-level concentration, while others contain a high-concentration. If you have a high-concentration reagent, you should keep it at the lower end of the pipette.
One reason to change the tip on a micropipetter is to keep the tip sterile. This will ensure that the reagents are not contaminated. You should also remember to use a tip ejector for sterile tips. When pipetting, you must have a lid. You should also never leave the tip on the bench while the liquids are in the pipettor.
In addition to the tips, micropipettes can be made with different materials. Some are graduated, while others are universal. The latter are designed to be sterile. A universal tip is designed to fit a variety of pipettes and is a good choice when transferring several different reagents. The reason to change the tip is that it will allow you to easily handle liquids with a high density or viscosity.
When transferring different reagents, it is important to use the correct tip. If you don't change the tip on a micropipette, you'll be wasting plastic. Moreover, you'll have to spend a lot of time comparing the two. You will want to get accurate results, but not worry about getting yourself stuck with the wrong tips.
Another reason to change the tip of micropipette is to avoid contamination. Often, a new micropipette is required to be used every time you transfer a different reagent. In some cases, you can reuse the same tip for two or three different reagents. A new tip may have been discarded.
After transferring different reagents, you should always change the tip on a micropipetter. This helps avoid contamination of stock reagents and helps you avoid contamination. You must also autoclave the micropipette tips and the pipette holders to ensure a sterile environment. And, as always, the tip should be adjusted before using.