One of the most common questions that we get from lab technicians is, "Why does alcohol drop from a pipette tip?" The answer to this question is actually very simple. There are a few things that you need to know about liquids before using one. The first thing that you need to understand is the concept of vapor pressure. Any liquid has an equilibrium between the two states and will continue to evaporate as long as the concentration is present in the surrounding atmosphere. Since acetone and acetonitrile are both vapors, they evaporate very quickly, which is why they are both so dangerous to use when working in a laboratory. Once the pipette tip fills with the liquid, you'll be able to observe the evaporation process as droplets hang from the tip.
Alternatively, you may wish to use a "to-contain" pipette. This type of pipette is useful for viscous liquids, such as agar. To avoid this, you can pre-wet the tip. This helps the air space inside reach an equilibrium point. However, this solution may not work for all types of liquids. You should always be sure to condition your pipettes first.
You can also try preparing your pipettes beforehand. Before starting any experiment, you should make sure that you are conditioned. Before using any sample, you should rinse the tip with the reagent several times. After the second rinse, you should be able to withdraw the reagent from the pipette without any problem. This will prevent the reagent from being diluted when the tip is removed.
In the lab, you can also check the calibration of your pipettes. Different types of pipettes have different markings on them. The leftmost one has markings every 0.1 mL, but it lacks intermediate markings. To deliver the liquid, you should squeeze the bulb to release it. Likewise, a to-contain pipette must be blown out with the pipette's bulb pressure.
The reason why alcohol drops from a pipette tip is due to the fact that liquids have different densities. Higher-density liquids tend to have more mass than lower-density ones. Thus, they are more dense than water and cause the liquid to drop from the pipette's tip. This results in a lower concentration. If the alcohol drips from the tip, the problem is exacerbated.
The most common type of pipette has a bulb that can be blown out. Its tip is used to transfer the liquid from the flask. The tubete tip is usually calibrated, but the bulb is not the only component that is important. It can have an arbitrary shape. Regardless, you should always check the measurements of your cylinders to be sure.
In most cases, the reagent is connected to the pipette tip. This means that the reagent is dissolved in the liquid, and the liquid is dispersed at a later stage.
Moreover, some liquids may be volatile. If you are using a pasteur pipete, you need to vacuum it first before using the sample. In order to avoid dilution, you must ensure that the tips do not break. It is not recommended to blow the tip of the pipette, since this can cause the volume to decrease. If the volume of the fluid drops too much, it could be due to a number of factors.
When it comes to liquids, the answer is often in the type of pipette. A pasteur pipete is specifically designed to deliver the liquid while a to-contain pipette is for containing it. A pasteur pipete has a double ring near the tip, and this is called a "to-deliver" pipete. In this case, the liquid is not delivered.