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Why Should You Never Blow Out the Liquid That is Caught in the Pipette Tip

Posted on March 26, 2022 by Pipette Tip 
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Volumetric pipettes should never be blown out, because they're calibrated to leave a small amount of liquid at the tip. Instead, you should hold the cap securely on the mouth of the pipette and allow the liquid to drain out of the bottom. This will ensure that you don't accidentally squish the remaining solution. In addition, you shouldn't use the pipette as a backscratcher or plunger. A well-calibrated volumetric tube will always have a little bit of liquid in its tip because it's calibrated for that volume.

Another reason why you shouldn't blow out the liquid that is caught in the pipette tip is because the surface tension of the liquid inside the container will assist in the process. If you don't blow out the liquid completely, you could risk spilling some of the liquid. A thin-walled tip will be more easily sucked out. It's a better idea to use a large-capacity bottle, which is also safer for the environment.

The last reason why you should never blow out the liquid that is trapped in the pipette tip is to prevent contamination. You can use the surface tension of the container to help with the process. If you don't have distilled water, you should rinse the pipette before you fill it. A solution or distilled water will help to remove any contaminated material from the tip.

If the tip is not attached to the pipette, you can use the air gap in the tip to draw out a larger volume. The tips should be installed with a small twist and without air gap. Slow aspiration will ensure that the liquid will be properly removed. Fast aspiration may result in bubbles and backlash, which could lead to inaccurate volumes. Cleanpipettes and the tips after every use.

To avoid backlash and bubbles, you should always make sure that the liquid is a few milliliters smaller than the volume of the pipette. This will prevent the pipette from drawing the correct amount of liquid. If you want to draw a bigger volume, then you need to use a thick-walled tip. If the tip is thin, you can press the end of the tip gently with your thumb.

Whether you're using a thin-walled tip or a thick-walled one, the volume delivered by a pipette varies. A thick-walled tip can deliver a larger volume, but a thin-walled orificed one can deliver more. By following these steps, you can use a pipette correctly.

If you're using a thin-walled pipette, the tip should be positioned properly to draw the liquid. If it isn't, it's a good idea to use a beveled tip. Its thin-walled design allows the liquid to escape. If the tip is thick, it will take longer to draw out.

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When you're working with liquids that are too thick to squeeze through a thin-walled tip, you can use a beveled tip. In addition, you can also pause before you blowout to make sure that the liquid is pushed through the pipette's surface tension. Be sure to clean the pipette before using it in the same container.

Besides a thin-walled tip, you should never try to blow out the liquid that is trapped inside a thick-walled tip. This can damage the pipette tip and may even cause it to break. Therefore, it's always better to pause before blowing out the liquid. If the pipette tip is thick, it can reduce the volume of the solution.

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