When using pipettes, new users are usually taught to always use a fresh tip. This practice creates a significant amount of waste, because the used tip ends up in the trash. However, the more savvy laboratory practitioner understands that there are certain instances when a used tip can be reused without any negative consequences. Here are a few of those situations.
Make sure the tip is attached securely, especially in the pipette shaft. Jamming can lead to broken pipette shafts. Additionally, the pipette shaft can easily get ruined if the tip jams. If you have a used tip, you should try to pre-wette it. A pre wet pipette tip is helpful. If this method does not work, the tips might break off, which could ruin your sample.
Changing the tip is also important when performing bench assays. During the procedure, you may notice that the tip is partially blocked by air, leading you to overcompensate by placing the tip deeper into the tube. In this case, the liquid enters the tip at a lower volume and results in underdelivery of the sample. This is not the ideal situation and should be avoided at all costs.
If you are using the micropipette to collect samples, you should always remember to replace the used tip before you put the sample into the tubes. The tip is usually inexpensive to replace, but it can be expensive to redo a whole experiment. This is why it's important to always use a new tip after every bench assay. It's much more efficient to avoid contamination of your samples by using the correct tip.
It's important to change the tip after each sample you've prepared in your lab. By doing so, you avoid cross-contamination from the used tip. In addition to cleaning the pipette, you should also change the tip after preparing the samples. You can use the same tip in the same experiments. Just make sure that you clean it before each pipetting.
Another reason to never set the pipette down with used tips on it is because you'll be unable to clean it properly with a used tip. In addition to avoiding contamination, pipettes should be cleaned thoroughly. Heavy use can cause parts to wear out and reduce the accuracy of liquid samples. As a result, it's best to ensure that the tips are clean before you start each bench assay.
In addition to the safety of your lab, you should also consider the contamination of the sample you're preparing in your experiments. Double-dipping occurs when you use the same tip to draw and dispense a different sample. This practice affects the downstream results and may result in contamination of both samples. Therefore, it's best to avoid double-dipping and use a new tip whenever you can.
The second reason to never set the pipette down with the used tip on it is that it is very easy to contaminate the sample. It is not only unsanitary, but it can also affect your lab equipment. When you want to prevent contamination, make sure you use a low-retention tip. A low retention tip repels the sample and prevents it from adhering to the pipette.
When you're pipetting, you need to use a new tip. In this way, the sample will be cleaner. If you use a used tip, you may end up contaminating your stock reagents. This can also cause multiple problems, such as contamination. If you use a new tip, you'll get the most accurate results.